A modified CAT expression vector with convenient cloning sites
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چکیده
منابع مشابه
A modified CAT expression vector with convenient cloning sites.
The chloramphenicol acetyl transferase (CAT) gene is widely used as a reporter gene to analyse promoters and other gene regulatory elements. Cloning of a DNA fragment into a CAT vector such as pSVO CAT (1) usually involves blunt-end ligation with no control over the orientation of the insert. This is because the first generation CAT vectors lack convenient multiple restriction sites immediately...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.7.1919